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Image Search Results
Journal: Asian Journal of Andrology
Article Title: Phosphoglycerate mutase 1 knockdown inhibits prostate cancer cell growth, migration, and invasion
doi: 10.4103/aja.aja_57_17
Figure Lengend Snippet: PGAM1 expression in prostate cancer tissues and prostate cancer cell lines. ( a ) The immunohistochemistry image of the whole TMA (scale bar = 1.5 mm). Representative immunohistochemistry images of PGAM1 protein expression from ( a ) with high intensity ( b ) in prostate cancer tissue (D7, scale bar = 0.2 mm), with intermediate intensity ( c ) in prostate cancer tissue (H7, scale bar = 0.2 mm), and with low intensity ( d ) in prostate cancer tissue (E4, scale bar = 0.2 mm). The percentage of PGAM1 positive cells in D7 is 90%, in H7 is 70%, and in E4 is 15%. Therefore, the percentage score of the case in D7 is 3 and its total protein expression score is 3 × 3 = 9. The percentage score of the case in H7 is 2 and its total protein expression score is 2 × 2 = 4. The percentage score of the case in E4 is 1 and its total protein expression score is 1 × 1 = 1. ( e ) Part of C14 from ( a ) with a magnification of 400× (scale bar = 50 μm). ( f ) Western blotting of PGAM1 protein expression in prostate cancer cell lines and normal prostate epithelial cell lines. PGAM1: phosphoglycerate mutase 1; TMA: tissue microarray.
Article Snippet: Four human PCa cell lines, PC-3, 22Rv1, DU145, and LNCap (ATCC, Manassas, VA, USA), and a
Techniques: Expressing, Immunohistochemistry, Western Blot, Microarray